Informations
In recent decades stem cell research has revolutionized the ways of curing. Till now, this possibility was available for only a little minority, despite the fact that everybody has his own „stem cell factory” and repository. In the future, not only privileged persons may benefit from this possibility of regeneration, but it has become available to anyone through a stem cell enhancer which is the result of numerous researches. tovább
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General information about stem cells

What is stem cell?

Stem cells are unspecialized cells that are able to go through unlimited cycles of cell division and self-renewals and by asymmetric replication are able to form cells identical to the original ones as well as to produce committed daughter cells.
The most important feature of stem cells is that they are able to transform themselves into any tissue cell, so we can call them general regeneration cells.

Following the conception the fertilized egg (ovum) begins to divide and through the uterine tube migrates towards the uterus and embedded there it is still a cell „capable of everything” (totipotent*). On the 15th day of the fetal development these cells begin to specialize into nerve cells, skin cells, muscle cell, etc. In these cells the different phases of the genetic material (DNA**) are activated and this is how the various organs develop, which, of course, have different functions in the human body.

The price of it is that the „capable of everything” cells lose their „capable of everything” feature and commit themselves, differentiate and incorporate into the body. But during the fetal development there remains some „capable of everything” cells that we call tissue (hematopoietic***) stem cells that can be derived from the umbilical cord blood just after the birth.

We distinguish two types of stem cells: embryonic stem cells and adult stem cells.
Embryonic stem cells can be derived from blastocysts which are formed during a 14 days cell division period following fertilization. Adult stem cells are found in our various tissues and are produced and stored in the bone marrow.

Adult and embryonic stem cells have very similar properties. Stem cells can be found in a number of tissues of the adult body. Tissue stem cells are present either in a continuously dividing or in a resting state.

Adult and embryonic stem cells have very similar properties. Stem cells can be found in a number of tissues of the adult body. Tissue stem cells are present either in a continuously dividing or in a resting state.

It is characteristic for vertebraes that the bone marrow, which is responsible for producing the most necessary cell types to maintain life – such as stem cells and red blood cells - can be found within the bone tissue, the most protected place in the body. Thus, negative external impacts are least able to influence the production of cells.

WHY THE NUMBER OF STEM CELLS AND REGENERATION ARE IMPORTANT?

If an organ does not work optimally or in some way suffers damage and needs cell replacement, it sends a message to the bone marrow that recognizes the information and triggers stem cells in the damaged area.
This is called the general regeneration mechanism of the body.

This process can be modelled as follows:

  • Stem cell leaves the bone marrow
  • Stem cell gets into the blood stream
  • Through a special messenger stem cell reaches the organs
  • When stem cell reaches the „target area” it uses its capability to divide, and forwards its replication, and the stem cell itself is integrated into the organ and becomes a cell of it.

As a result, the organ will be able to regenerate and to function in a healthy way while retaining its functions. This process occurs several billion times in our bodies daily because our body achieves regeneration and renewal by general cell replacement.

This process makes possible the renewal of skin tissue, the healing of wounds, the fusing of bones and this process also supports metabolic processes and detoxification.

Without constant replacement of the cells the organs and tissues of our body would wear out in a few weeks and they would cease to function. The number of stem cells circulating in the bloodstream shows the highest value at our birth. From the age of 25-30 years (depending on lifestyle and nutrition) this number begins to decline gradually and by the time we reach the retirement age it is only one tenth of what was at birth.

It can be said that the decline in the number of stem cells is an accurate physiological indicator of lack of regeneration, that is of aging.

A great number of studies have shown that in those with higher circulating stem cells the healing of tissues is faster (easier to build up from myocardial infarction), their immune system is stronger, they are more resistant to age-related degenerative disorders and bone fractures occur less frequently. Taken together both their mental and physical performance is better.

So it can be stated that the number of stem cells is a physiological indicator that is able to provide information on overall health condition of the body.

STEM CELL ENHANCER

In case of diabetes

Hungarian researchers artificially damaged the so called insulin producing beta cells in the pancreas of the experimental animals, then one group was fed with a stem cell enhancer product in addition to their normal diet.

Figure 1 well illustrates that insulin production improved significantly in animals consuming special stem cell enhancer product compared to the control group.
It is probable that the damaged tissue cells were replaced by the additionally produced stem cells, which restored insulin production.

Department of Pharmacology, University of Medicine and Pharmacy Targu Mures, 2. Ophtalmology Clinic, Targu Mures, 3. Department of Pathology, University of Medicine and Pharmacy, Targu Mures, 4. Department of Public Health, University of Pecs, Hungary, 5. Crystal Institute KFT, Eger, Hungary. Doczi-Keresztesi Z. 1,2, Jung J. 3, Kiss I. 4, Mezei T. 3 Szabo L. 5, Ember I. 4

THE SUBJECT OF THE STUDY:
To determine whether treatment with a special stem cell enhancer preparation, a long-term cell stimulator substance can prevent the increase of hyperglycemia induced retinal and renal vessel permeability, and whether it can inhibit the blood-retinal barrier breakdown (BRB) in rats with alloxane-induced* diabetes.

METHODS:
Male Whistar rats were divided into three groups. Two of the groups received a single intraperitoneal injection of Alloxan (125 mg/kg) – a toxic substance which destroys insulin producing beta cells in pancreas; the third group received vehicle.

One group of the alloxan induced diabetic rats was treated with a special stem cell enhancer for 4 weeks, while the diabetic group not treated with stem cell and the non-diabetic control group was fed by standard laboratory feed. The blood-retinal barrier and the renal vessel’s permeability were measured by fluorescein isothiocyanate* leakage that was labeled by bovine serum albumin* (FITC-BSA).

*Alloxan: oxidation product of uric acid, inducing diabetes in experimental animals by damaging the islets of Langerhans of pancreas
**Fluorescein isothiocyanate: fluorochrome stain often linked to antibodies so the corresponding antigens can be detected and localized
***Serum albumin: a protein in the blood plasma

RESULTS:
Following a six-week treatment with alloxan significant tissue fluorescence, renal vessel leakage and BRB breakdown occured in the diabetic group compared with non-diabetic group. Treatment with stem cell enhancer reduced BRB breakdown, tissue fluorescence, and vessel leakage significantly, compared to the untreated group.

The mechanism of these effects may include the regeneration of the vessel system (endothelium), which is induced by stem cell stimulation. Blood glucose values decreased gradually without showing significant differences among groups thus regeneration of insulin-secreting beta cell could not be detected.

CONCLUSION:
The results of the study suggest that the special stem cell enhancer product may be useful in the treatment of diabetes complications such as nephropathy and retinopathy, which are associated with increased vessel permeability.

Means and distribution of circulating CD34+* cells before administering stem cell enhancer and after 2 hours of administration
The fluctuation in the number of stem cells following consumption of coffe after 1, 3 and 6 hours
 
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